Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
نویسندگان
چکیده
منابع مشابه
Combinatorial approaches for inverse metabolic engineering applications
Traditional metabolic engineering analyzes biosynthetic and physiological pathways, identifies bottlenecks, and makes targeted genetic modifications with the ultimate goal of increasing the production of high-value products in living cells. Such efforts have led to the development of a variety of organisms with industrially relevant properties. However, there are a number of cellular phenotypes...
متن کاملCorynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi).
Corynebacterium glutamicum is an important organism for the industrial production of amino acids. Metabolic pathways in this organism are usually engineered by conventional methods such as homologous recombination, which depends on rare double-crossover events. To facilitate the mapping of gene expression levels to metabolic outputs, we applied CRISPR interference (CRISPRi) technology using dea...
متن کاملMultiplex genome engineering using CRISPR/Cas systems.
Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be dire...
متن کاملA Functional Protein Chip for Combinatorial Pathway Optimization and In Vitro Metabolic Engineering
Pathway optimization is, in general, a very demanding task due to the complex, nonlinear and largely unknown interactions of enzymes, regulators and metabolites. While in vitro reconstruction and pathway analysis is a viable alternative, a major limitation of this approach is the availability of the pathway enzymes for reliable pathway reconstruction. Here, we report the application of RNA disp...
متن کاملCombinatorial Orthogonal Expansions
so that amn is non-negative. In this paper we give in Theorem 1 and Theorem 2 explicit formulas for anm as a polynomial in the α ′ js, β ′ js and the γ ′ js, which give these theorems. The idea is to represent amn as a generating function of paths, whose weights are products of differences. Monotonicity hypotheses on the coefficients force the weights to be individually positive, these are the ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Communications
سال: 2017
ISSN: 2041-1723
DOI: 10.1038/s41467-017-01695-x